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Evaluation of a Real-Time PCR Assay Using the LightCycler System for Detection of Parvovirus B19 DNA

2004; American Society for Microbiology; Volume: 42; Issue: 7 Linguagem: Inglês

10.1128/jcm.42.7.3326-3328.2004

1098-660X

Richard S. Buller, Gregory A. Storch,

Prenatal Screening and Diagnostics

We evaluated the artus RealArt Parvovirus B19 LC PCR reagent (artus biotech USA, San Francisco, Calif.) for real-time PCR detection of parvovirus B19 DNA by retesting 71 specimens previously submitted to our laboratory. The artus assay, which produces a quantitative result and provides an internal PCR control, appeared to be slightly more sensitive than our conventional qualitative PCR assay.