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Deoxyribonucleic Acid Relatedness between Selected Members of the Genus Aquaspirillum by Slot Blot Hybridization: Aquaspirillum serpens (Mueller 1786) Hylemon, Wells, Krieg, and Jannasch 1973 Emended to Include Aquaspirillum bengal as a Subjective Synonym

1985; Microbiology Society; Volume: 35; Issue: 4 Linguagem: Inglês

10.1099/00207713-35-4-512

1465-2102

Myriam Boivin, V L Morris, Edwin Lee‐Chan, R. G. E. Murray,

Plant-Microbe Interactions and Immunity

Deoxyribonucleic acid relatedness of five strains of Aquaspirillum serpens and also four other species of this genus was assessed by slot blot hybridization. A high degree of genetic relatedness was evident among the strains within the species A. serpens, but a low degree of relatedness was shown between the species A. serpens, A. sinuosum, A. itersonii, and A. putridiconchylium. Hybridization homology indicated that A. bengal is a subjective synonym for A. serpens and should not have separate species status; therefore, the description of A. serpens is emended accordingly. The strains that grow optimally at higher temperatures (41°C) and produce brown pigments in the presence of some amino acids may be considered varietal and are referred to as A. serpens biovar bengal. This study demonstrates the usefulness of slot blot hybridization for quickly assessing the relatedness of different taxonomic groups of bacteria.