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Localization of Cathepsin K and Tartrate‐Resistant Acid Phosphatase in Synovium and Cranial Cruciate Ligament in Dogs with Cruciate Disease

2005; Wiley; Volume: 34; Issue: 3 Linguagem: Inglês

10.1111/j.1532-950x.2005.00036.x

1532-950X

Peter Muir, Gwenn M. Schamberger, Paul A. Manley, Zhengling Hao,

Tendon Structure and Treatment

Objective— To localize cathepsin K and tartrate‐resistant acid phosphatase (TRAP) in synovium and cranial cruciate ligament (CCL) of dogs with cruciate disease. Animals— Dogs (n=15) with cruciate disease and ruptured CCL, and 12 dogs with intact CCL. Methods— Synovium and CCL were examined histologically and cells containing cathepsin K or TRAP were identified immunohistochemically and histochemically, respectively. Results— Increased cellular localization of cathepsin K and TRAP was detected in synovium and ruptured CCL in dogs with cruciate disease, when compared with tissues from dogs with intact CCL. Inflammation of synovium with TRAP + macrophage‐like cells was seen in 73% of dogs with CCL disease, but was not seen in dogs with intact CCL. The presence of cathepsin K and TRAP protein in synovium and CCL tissues was significantly correlated in dogs with CCL rupture. Conclusion— Inflammation of the epiligament of ruptured CCL with cathepsin K + and TRAP + macrophage‐like cells forms part of a similar, more generalized chronic inflammatory change within the periarticular tissues of the stifle of a large proportion of dogs with CCL rupture. Clinical Relevance— Production of matrix‐degrading enzymes by the synovium may induce progressive pathologic rupture of the CCL. Therefore, these collagenolytic pathways may offer a novel target for medical therapy of joint inflammation in canine patients with cruciate disease.