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sn -Glycerol-1-Phosphate-Forming Activities in Archaea : Separation of Archaeal Phospholipid Biosynthesis and Glycerol Catabolism by Glycerophosphate Enantiomers

1999; American Society for Microbiology; Volume: 181; Issue: 4 Linguagem: Inglês

10.1128/jb.181.4.1330-1333.1999

1098-5530

Masateru Nishihara, Tomoko Yamazaki, Tairo Oshima, Yosuke Koga,

Metabolism and Genetic Disorders

ABSTRACT In Methanobacterium thermoautotrophicum , sn -glycerol-1-phosphate (G-1-P) dehydrogenase is responsible for the formation of the Archaea -specific backbone of phospholipids, G-1-P, from dihydroxyacetonephosphate (DHAP). The possible G-1-P-forming activities were surveyed in cell-free extracts of six species of Archaea . All the archaeal cell-free homogenates tested revealed the ability to form G-1-P from DHAP. In addition, activities of G-3-P-forming glycerol kinase and G-3-P dehydrogenase were also detected in four heterotrophic archaea, while glycerol kinase activity was not detected in two autotrophic methanogens. These results show that G-1-P is produced from DHAP by G-1-P dehydrogenase in a wide variety of archaea while exogenous glycerol is catabolized via G-3-P.