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BIBTEX RIS

A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine

2012; American Society for Microbiology; Volume: 50; Issue: 6 Linguagem: Inglês

10.1128/jcm.00481-12

ISSN

1098-660X

Autores

Nobuo Koizumi, Chie Nakajima, Tsunehito Harunari, Tsutomu Tanikawa, Toshihiro Tokiwa, Eriko Uchimura, Tokujiro Furuya, Claro N. Mingala, Marvin A. Villanueva, Makoto Ohnishi, Yasuhiko Suzuki,

Tópico(s)

Viral gastroenteritis research and epidemiology

Resumo

ABSTRACT We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs , a 16S rRNA gene of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB nested PCR.

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