Detection of anti-ESA antibodies in human samples from PRCA and non-PRCA patients: an immunoassay platform comparison
2011; Oxford University Press; Volume: 27; Issue: 2 Linguagem: Inglês
10.1093/ndt/gfr213
ISSN1460-2385
AutoresTroy E. Barger, Andrew J. Kuck, Narendra Chirmule, Steven J. Swanson, Daniel T. Mytych,
Tópico(s)Monoclonal and Polyclonal Antibodies Research
ResumoBackground.The immunological methods for detecting antibodies to erythropoiesis-stimulating agents (ESAs) differ in assay sensitivity. However, this parameter, routinely determined in clinical assays using a high-affinity non-human polyclonal antibody, gives a one-dimensional assessment of antibody detection. We compare three widely used immunological methods and evaluate the ability of each to detect mature human antibodies and human antibodies characteristic of an early immune response.
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