Co-Transplantation of Endothelial Progenitor Cells and Mesenchymal Stem Cells Promote Neovascularization and Bone Regeneration
2013; Wiley; Volume: 17; Issue: 2 Linguagem: Inglês
10.1111/cid.12104
ISSN1708-8208
AutoresHadar Zigdon‐Giladi, Tova Bick, Dina Lewinson, Eli E. Machtei,
Tópico(s)Periodontal Regeneration and Treatments
ResumoClinical Implant Dentistry and Related ResearchVolume 17, Issue 2 p. 353-359 ARTICLE Co-Transplantation of Endothelial Progenitor Cells and Mesenchymal Stem Cells Promote Neovascularization and Bone Regeneration Hadar Zigdon-Giladi DMD, PhD, Corresponding Author Hadar Zigdon-Giladi DMD, PhD Department of Periodontology, School of Graduate Dentistry, Rambam Health Care Campus, Haifa, Israel Research Institute for Bone Repair, Rambam Health Care Campus, Haifa, Israel The Rappaport Family Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, IsraelReprint requests: Dr. Hadar Zigdon-Giladi, Research Institute for Bone Repair, Rambam Health Care Campus, 8, Ha'liya st., Bat-Galim, P.O. Box 9602, Haifa 31096, Israel; e-mail: [email protected]Search for more papers by this authorTova Bick PhD, Tova Bick PhD Research Institute for Bone Repair, Rambam Health Care Campus, Haifa, IsraelSearch for more papers by this authorDina Lewinson PhD, Dina Lewinson PhD Research Institute for Bone Repair, Rambam Health Care Campus, Haifa, IsraelSearch for more papers by this authorEli E. Machtei DMD, Eli E. Machtei DMD associate professor Department of Periodontology, School of Graduate Dentistry, Rambam Health Care Campus, Haifa, Israel The Rappaport Family Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, IsraelSearch for more papers by this author Hadar Zigdon-Giladi DMD, PhD, Corresponding Author Hadar Zigdon-Giladi DMD, PhD Department of Periodontology, School of Graduate Dentistry, Rambam Health Care Campus, Haifa, Israel Research Institute for Bone Repair, Rambam Health Care Campus, Haifa, Israel The Rappaport Family Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, IsraelReprint requests: Dr. Hadar Zigdon-Giladi, Research Institute for Bone Repair, Rambam Health Care Campus, 8, Ha'liya st., Bat-Galim, P.O. Box 9602, Haifa 31096, Israel; e-mail: [email protected]Search for more papers by this authorTova Bick PhD, Tova Bick PhD Research Institute for Bone Repair, Rambam Health Care Campus, Haifa, IsraelSearch for more papers by this authorDina Lewinson PhD, Dina Lewinson PhD Research Institute for Bone Repair, Rambam Health Care Campus, Haifa, IsraelSearch for more papers by this authorEli E. Machtei DMD, Eli E. Machtei DMD associate professor Department of Periodontology, School of Graduate Dentistry, Rambam Health Care Campus, Haifa, Israel The Rappaport Family Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, IsraelSearch for more papers by this author First published: 12 July 2013 https://doi.org/10.1111/cid.12104Citations: 34Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Background Bone formation relies on sufficient blood supply and osteoprogenitor cells. Purpose The study aims to evaluate the influence of endothelial progenitor cells (EPCs) in combination with mesenchymal stem cells (MSCs) on early vascularization and intramembranous bone regeneration. Materials and Methods Vertical bone regeneration was tested in rat calvarium guided bone regeneration model. Gold domes were filled with a mixture of 5 × 105 osteogenic transformed MSC and 5 × 105 EPC (EPC/MSC) that were mixed with β-tricalcium phosphate (βTCP) scaffold. Domes filled with βTCP alone served as control. Rats were sacrificed after 4 or 12 weeks. Histomorphometry was used to determine blood vessel (Bv) density, vertical bone height, and bone area in the regenerated tissue. Results At both time points, new augmented hard tissue filled the space under the dome, and Bv density was higher in the EPC/MSC transplanted group vs control. 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