Activación de la Calcio Calmodulina quinasa II. CaMKII, por el uridin nucleósido difosfato, UDP, en neuronas granulares de cerebelo
2005; Royal Academy of Pharmacy; Volume: 71; Issue: 2 Linguagem: Inglês
ISSN
2174-8756
AutoresCristina Hervás, David León, Raquel P. Sen, María Teresa Miras Portugal,
Tópico(s)Ion channel regulation and function
ResumoCultured granule cells from cerebellum exhibit nucleotide metabotropic receptors such as the P2Y6 subtype, which physiological agonist is the uridine diphosphate, UDP. The PCR analysis show the presence of P2Y6 messenger RNA, increasing with the days in culture. Single cell microfluorimetric studies show citosolic calcium increase in response to UDP, this being more significant at the soma level. The cytosolic calcium increase triggers cellular responses mediated by calcium dependent enzymes. This is the case for calcium-calmodulin kinase II, CaMKII, which is extensively distributed through the granule neuron, according the immunocytochemical studies. This enzyme once activated is able to autophosphorylate and by using antibodies against the phosphorylated form the active zones can be detected. After UDP stimulation, the location of the phosphorylated form of CaMKII appears to be mainly at the neural soma, with lower presence at the axodendritic prolongations, which correlates with the functional P2Y6 subtype receptor distribution.
Referência(s)