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Cellulophaga geojensis sp. nov., a member of the family Flavobacteriaceae isolated from marine sand

2011; Microbiology Society; Volume: 62; Issue: Pt_6 Linguagem: Inglês

10.1099/ijs.0.033340-0

1466-5034

Sooyeon Park, Ki‐Hoon Oh, Soo Young Lee, Tae-Kwang Oh, Jung‐Hoon Yoon,

Molecular Biology Techniques and Applications

A Gram-stain-negative, aerobic, non-flagellated, non-spore-forming, motile (by gliding) bacterial strain, designated M-M6 T , was isolated from marine sand of Geoje island, Korea. Strain M-M6 T grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain M-M6 T fell within the clade comprising Cellulophaga species, forming a coherent cluster with Cellulophaga lytica ATCC 23178 T and Cellulophaga fucicola NN015860 T , with which it shared 16S rRNA gene sequence similarities of 98.1 and 98.2 %, respectively. Sequence similarities between strain M-M6 T and the type strains of other recognized Cellulophaga species were in the range 92.4–93.8 %. Strain M-M6 T contained MK-6 as the predominant menaquinone and iso-C 15 : 0 , iso-C 15 : 1 G, iso-C 17 : 0 3-OH, and C 16 : 1 ω7 c and/or iso-C 15 : 0 2-OH as the major fatty acids. The major polar lipids detected in strain M-M6 T and the type strains of C. lytica and C. fucicola were two unidentified lipids, one unidentified aminolipid and one unidentified aminophospholipid. The DNA G+C content of strain M-M6 T was 35.4 mol%. Levels of DNA–DNA relatedness between strain M-M6 T and C. lytica JCM 8516 T and C. fucicola JCM 21778 T were 33 and 35 %, respectively. Differential phenotypic properties and phylogenetic and genetic distinctiveness distinguished strain M-M6 T from all recognized Cellulophaga species. On the basis of the data presented, strain M-M6 T is considered to represent a novel species of the genus Cellulophaga , for which the name Cellulophaga geojensis sp. nov. is proposed. The type strain is M-M6 T ( = KCTC 23498 T = CCUG 60801 T ).