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Flow Cytometric Immunophenotypic Characterization of Pediatric and Adult Minimally Differentiated Acute Myeloid Leukemia (AML-M0)

2000; Oxford University Press; Volume: 113; Issue: 2 Linguagem: Inglês

10.1309/2fyj-00be-r8n0-hmpq

1943-7722

Patricia K. Kotylo, In-Sook Seo, Franklin O. Smith, Nyla A. Heerema, Naomi S. Fineberg, Kathy D. Miller, Marianne E. Greene, Pauline M. Chou, Attilio Orazi,

Myeloproliferative Neoplasms: Diagnosis and Treatment

We reviewed the clinicopathologic and immunophenotypic profiles of 7 pediatric and 11 adult minimally differentiated acute myelogenous leukemias (AML-M0). We also compared and evaluated myeloperoxidase in leukemic blasts using standard cytochemical and polyclonal antibody immunohistochemical stains. No distinctive clinical findings were noted in either patient group; however, thrombocytopenia typically was more prominent in adults. Adult AML-M0 also was associated with an immature myeloid profile (CD34+, terminal deoxynucleotidyl transferase positive, CD13+, and CD33+), in contrast with pediatric AML-M0, which usually lacked terminal deoxynucleotidyl transferase or CD34 but expressed bright CD33 with weak or negative CD13. Coexpression of the T-cell-associated antigen CD7 was observed in both groups. Antibody immunohistochemical stains were more sensitive than cytochemical stains for detection of myeloperoxidase activity and a useful adjunct for establishing a diagnosis of myeloid leukemia in paraffin-embedded marrow tissues.