Effect of diets containing a purified soybean trypsin inhibitor on growth performance, digestive proteases and intestinal histology in juvenile sea bream (Sparus aurata L.)
2010; Wiley; Volume: 41; Issue: 9 Linguagem: Inglês
10.1111/j.1365-2109.2010.02500.x
ISSN1365-2109
AutoresEster Santigosa, M. Sáenz de Rodrigáñez, Ana Rodiles, Fernando G. Barroso, Francisco Javier Alarcón,
Tópico(s)Reproductive biology and impacts on aquatic species
ResumoAquaculture ResearchVolume 41, Issue 9 p. e187-e198 Effect of diets containing a purified soybean trypsin inhibitor on growth performance, digestive proteases and intestinal histology in juvenile sea bream (Sparus aurata L.) Ester Santigosa, Ester Santigosa Department of Physiology, Faculty of Biology, University of Barcelona, Barcelona, Spain * Present address: E Santigosa, Ifremer, UMR 1067 Fish Nutrition Unit, BP 70, 29280 Plouzane, France.Search for more papers by this authorMiguel Ángel Sáenz de Rodrigáñez, Miguel Ángel Sáenz de Rodrigáñez Department of Applied Biology, University of Almería, Almería, SpainSearch for more papers by this authorAna Rodiles, Ana Rodiles IFAPA Aguas del Pino, Cartaya-Punta Umbría, Huelva, SpainSearch for more papers by this authorFernando García Barroso, Fernando García Barroso Department of Applied Biology, University of Almería, Almería, SpainSearch for more papers by this authorFrancisco Javier Alarcón, Francisco Javier Alarcón Department of Applied Biology, University of Almería, Almería, SpainSearch for more papers by this author Ester Santigosa, Ester Santigosa Department of Physiology, Faculty of Biology, University of Barcelona, Barcelona, Spain * Present address: E Santigosa, Ifremer, UMR 1067 Fish Nutrition Unit, BP 70, 29280 Plouzane, France.Search for more papers by this authorMiguel Ángel Sáenz de Rodrigáñez, Miguel Ángel Sáenz de Rodrigáñez Department of Applied Biology, University of Almería, Almería, SpainSearch for more papers by this authorAna Rodiles, Ana Rodiles IFAPA Aguas del Pino, Cartaya-Punta Umbría, Huelva, SpainSearch for more papers by this authorFernando García Barroso, Fernando García Barroso Department of Applied Biology, University of Almería, Almería, SpainSearch for more papers by this authorFrancisco Javier Alarcón, Francisco Javier Alarcón Department of Applied Biology, University of Almería, Almería, SpainSearch for more papers by this author First published: 19 August 2010 https://doi.org/10.1111/j.1365-2109.2010.02500.xCitations: 17 Correspondence: F J Alarcón, Department of Applied Biology, University of Almería, 04120 Almería, Spain. E-mail: [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Juvenile sea bream were fed on diets containing 0.0, 2.0 or 4.0 g kg−1 of a soybean trypsin inhibitor (SBTI) for 30 days. The growth performance, total protease activity and intestinal histology were studied after 0, 15 and 30 days of dietary treatment. No significant differences were found in the weight gain, specific growth rate (SGR) and feed conversion rate in fish fed on inhibitor-supplemented diets when compared with those fed on an inhibitor-free diet. Only the SGR at day 15 decreased significantly with protease inhibitor inclusion, although this effect was not observed at day 30. In relation to proteolytic activity at day 15, the total protease activity in the distal intestine decreased in fish fed on inhibitor-supplemented diets. Zymograms of these extracts showed that the SBTI reduced the intensity of some proteolytic fractions in the distal intestine. A noticeable reduction in the protease activity of the intestinal content in fish fed on the highest level of soybean inhibitor (4.0 g kg−1) was also observed. However, at day 30, the inhibition effect on these active bands was not detected, and the total protease activity was similar to that in fish fed on an inhibitor-free diet. Histological examination revealed no perceptible differences in the intestinal structure between any of the diet groups. 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