Tissue Culture Studies on Murine Virus-induced Leukemia Cells: Isolation of Single Cells in Agar-Liquid Medium
1967; American Association for Cancer Research; Volume: 27; Issue: 4 Linguagem: Inglês
ISSN
1538-7445
AutoresMaria C. Patuleia, Charlotte Friend,
Tópico(s)Erythrocyte Function and Pathophysiology
ResumoSummary Maturation along the erythrocytic line is observed in long-term cell cultures of murine virus-induced leukemic cells. In attempts to determine the relationship between the different morphologic types in these cultures, an adaptation of the Sanders technic of plating and cloning cells utilizing an agar-liquid medium was devised. This method permitted the cloning of single cells. The progeny of each clone consisted of primitive cells, proerythroblasts, and erythroblasts. From these data the possibility is considered that the primitive cell characteristic of the murine leukemia under study is an erythrocyte precursor.
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