Artigo Revisado por pares

Reverse engineering of long‐chain polyunsaturated fatty acid biosynthesis into transgenic plants

2005; Wiley; Volume: 107; Issue: 4 Linguagem: Inglês

10.1002/ejlt.200590022

ISSN

1438-9312

Autores

Johnathan A. Napier, Frédéric Beaudoin, Olga Sayanova,

Tópico(s)

Nitrogen and Sulfur Effects on Brassica

Resumo

European Journal of Lipid Science and TechnologyVolume 107, Issue 4 p. 249-255 Dossier Reverse engineering of long-chain polyunsaturated fatty acid biosynthesis into transgenic plants Johnathan A. Napier, Corresponding Author Johnathan A. Napier [email protected] Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Herts, UKCrop Performance and Improvement Division, Rothamsted Research, Harpenden, Herts AL5 2JQ, UK. Phone: +44 1582 763133, Fax: +44 1582 763010Search for more papers by this authorFrédéric Beaudoin, Frédéric Beaudoin Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Herts, UKSearch for more papers by this authorOlga Sayanova, Olga Sayanova Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Herts, UKSearch for more papers by this author Johnathan A. Napier, Corresponding Author Johnathan A. Napier [email protected] Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Herts, UKCrop Performance and Improvement Division, Rothamsted Research, Harpenden, Herts AL5 2JQ, UK. Phone: +44 1582 763133, Fax: +44 1582 763010Search for more papers by this authorFrédéric Beaudoin, Frédéric Beaudoin Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Herts, UKSearch for more papers by this authorOlga Sayanova, Olga Sayanova Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Herts, UKSearch for more papers by this author First published: 15 April 2005 https://doi.org/10.1002/ejlt.200590022Citations: 7AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat References 1 J. A. Napier: The production of long chain polyunsaturated fatty acids in transgenic plants: a sustainable source for human health and nutrition. Lipid Technol. 2004, 16, 103–107. 2 J. G. Wallis, J. L. Watts, J. Browse: Polyunsaturated fatty acid synthesis: what will they think of next? Trends Biochem Sci. 2002, 27, 467–470. 3 O. Sayanova, J. A. Napier: Eicosapentaenoic acid: biosynthetic routes and the potential for synthesis in transgenic plants. Phytochemistry. 2004, 65, 147–58. 4 A. P. Nugent: The metabolic syndrome. Nutrition Bull. 2004, 29, 36–43. 5 J. J. Thelen, J. B. Ohlrogge: Metabolic engineering of fatty acid biosynthesis in plants. Metab Eng. 2002, 4, 12–21. 6 J. A. Napier, L. V. Michaelson, O. Sayanova: The role of cytochrome b5 fusion desaturases in the synthesis of polyunsaturated fatty acids. Prostaglandins Leukot Essent Fatty Acids. 2003, 68, 135–143. 7 P. Sperling, P. Ternes, T. K. Zank, E. Heinz: The evolution of desaturases. Prostaglandins Leukot Essent Fatty Acids. 2003, 68, 73–95. 8 F. Beaudoin, L. V. Michaelson, S. J. Hey, M. J. Lewis, P. R. Shewry, O. Sayanova, J. A. Napier: Heterologous reconstitution in yeast of the polyunsaturated fatty acid biosynthetic pathway. Proc Natl Acad Sci USA. 2000, 97, 6421–6426. 9 J. M. Parker-Barnes, T. Das, E. Bobik, A. E. Leonard, J. M. Thurmond, L. T. Chaung, Y. S. Huang, P. Mukerji: Identification and characterization of an enzyme involved in the elongation of n-6 and n-3 polyunsaturated fatty acids. Proc Natl Acad Sci USA. 2000, 97, 8284–8289. 10 A. A. Millar, L. Kunst: Very-long-chain fatty acid biosynthesis is controlled through the expression and specificity of the condensing enzyme. Plant J. 1997, 12, 121–131. 11 A. Abbadi, F. Domergue, A. Meyer, K. Riedel, P. Sperling, T. Zank, E. Heinz: Transgenic oilseeds as sustainable source of nutritionally relevant C20 and C22 polyunsaturated fatty acids? Eur J Lipid Sci Technol. 2001, 103, 106–113. 12 J. G. Metz, P. Roessler, D. Facciotti, C. Levering, F. Dittrich, M. Lassner, R. Valentine, K. Lardizabal, F. Domergue, A. Yamada, K. Yazawa, V. Knauf, J. Browse: Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science. 2001, 293, 290–293. 13 J. A. Napier: Plumbing the depths of PUFA biosynthesis: a novel polyketide synthase-like pathway from marine organisms. Trends Plant Sci. 2002, 7, 51–54. 14 F. Domergue, J. Lerchl, U. Zähringer, E. Heinz: Cloning and functional characterization of Phaeodactylum tricornutum front-end desaturases involved in eicosapentaenoic acid biosynthesis. Eur J Biochem. 2002, 269, 4105–4113. 15 H. Drexler, P. Spiekermann, A. Meyer, F. Domergue, T. Zank, P. Sperling, A. Abbadi, E. Heinz: Metabolic engineering of fatty acids for breeding of new oilseed crops: strategies, problems and first results. J Plant Physiol. 2001, 160, 779–802. 16 F. Domergue, A. Abbadi, C. Ott, T. K. Zank, U. Zähringer, E. Heinz: Acyl carriers used as substrates by the desaturases and elongases involved in very long-chain polyunsaturated fatty acids biosynthesis reconstituted in yeast. J Biol Chem. 2003, 278, 35115–35126. 17 B. Qi, F. Beaudoin, T. Fraser, A. K. Stobart, J. A. Napier, C. M. Lazarus: Identification of a cDNA encoding a novel C18-Δ9 polyunsaturated fatty acid-specific elongating activity from the docosahexaenoic acid (DHA)-producing microalga, Isochrysis galbana. FEBS Lett. 2002, 510, 159–165. 18 J. G. Wallis, J. Browse: The Δ8-desaturase of Euglena gracilis: an alternate pathway for synthesis of 20-carbon polyunsaturated fatty acids. Arch Biochem Biophys. 1999, 365, 307–316. 19 T. C. Fraser, B. Qi, S. Elhussein, S. Chatrattanakunchai, A. K. Stobart, C. M. Lazarus: Expression of the Isochrysis C18-Δ9 polyunsaturated fatty acid specific elongase component alters Arabidopsis glycerolipid profiles. Plant Physiol. 2004, 135, 859–866. 20 A. A. Millar, M. Wrischer, L. Kunst: Accumulation of very-long-chain fatty acids in membrane glycerolipids is associated with dramatic alterations in plant morphology. Plant Cell. 1998, 10, 1889–1902. 21 B. Qi, T. Fraser, S. Mugford, G. Dobson, O. Sayanova, J. Butler, J. Napier, A. K. Stobart, C. M. Lazarus: The production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in transgenic plants. Nature Biotechnol. 2004, 22, 739–745. 22 D. S. Knutzon, J. M. Thurmond, Y. S. Huang, S. Chaudhary, E. G. Bobik Jr., G. M. Chan, S. J. Kirchner, P. Mukerji: Identification of Δ5-desaturase from Mortierella alpina by heterologous expression in Bakers' yeast and canola. J Biol Chem. 1998, 273, 29360–29366. 23 S. Nakane, T. Tanaka, K. Satouchi, Y. Kobayashi, K. Waku, T. Sugiura: Occurrence of a novel cannabimimetic molecule 2-sciadonoylglycerol (2-eicosa-5',11',14'-trienoylglycerol) in the umbrella pine Sciadopitys verticillata seeds. Biol Pharm Bull. 2000, 23, 758–761. 24 A. G. Green: From alpha to omega-producing essential fatty acids in plants. Nature Biotechnol. 2004, 22, 680–682. 25 A. Abbadi, F. Domergue, A. Fahl, C. Ott, J. Bauer, J. A. Napier, R. Welti, P. Cirpus, E. Heinz: Biosynthesis of very long chain polyunsaturated fatty acids in transgenic oilseeds: constraints on their accumulation. Plant Cell. 2004, 16, 2734–2748. 26 T. J. Roscoe: Identification of acyltransferases controlling triacylglycerol biosynthesis in oilseeds using a genomics-based approach. Eur J Lipid Sci Technol. 2005, 107, 256–262. 27 F. Beaudoin, J. A. Napier: Biosynthesis and compartmentalisation of triacyglycerol in higher plants. Topics Curr Genetics. 2004, 6, 267–287. 28 A. Meyer, H. Kirsch, F. Domergue, A. Abbadi, P. Sperling, J. Bauer, P. Cirpus, T. K. Zank, H. Moreau, T. J. Roscoe, U. Zähringer, E. Heinz: Novel fatty acid elongases and their use for the reconstitution of docosahexaenoic acid biosynthesis. J Lipid Res. 2004, 45, 1899–1909. 29 S. L. Pereira, A. E. Leonard, Y. S. Huang, L. T. Chuang, P. Mukerji: Identification of two novel microalgal enzymes involved in the conversion of the omega-fatty acid, eicosapentaenoic acid, into docosahexaenoic acid. Biochem J. 2004, 384, 357–366. 30 H.-G. Opsahl-Ferstad, H. Rudi, B. Ruyter, S. Refstie: Biotechnological approaches to modify rapeseed oil composition for applications in aquaculture. Plant Sci. 2003, 165, 349–357. Citing Literature Volume107, Issue4No. 4 April 2005Pages 249-255 ReferencesRelatedInformation

Referência(s)