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In vitro effects of high glucose concentrations on membrane protein oxidation, G‐actin and deformability of human erythrocytes

2004; Wiley; Volume: 23; Issue: 3 Linguagem: Inglês

10.1002/cbf.1129

1099-0844

Halil Resmi, Hülya Akhunlar, Ayşegül Temiz Artmann, Gül Güner,

Diabetes Management and Research

Abstract The object of this study was to examine the effect of elevated in vitro glucose concentrations on protein modification and functional changes in human erythrocytes. Groups were exposed to 5–45 m M glucose concentrations. The time effect of any changes was also evaluated. In erythrocyte ghosts, protein glycation and oxidation were evaluated using spectrophotometric methods. G‐actin was measured by a DNase I inhibition assay in cell lysates. Erythrocyte deformability was assessed using a cell transit analyser. At 24 h, a significant protein oxidation (at 25 and 45 m M glucose; p < 0.05), and G‐actin increase was observed for all concentrations ( p < 0.05). At 48 h, a significant increase in glycation (25 and 45 m M glucose; p < 0.05), protein oxidation ( p < 0.05), and G‐actin ( p < 0.05) was observed in all groups. A significant positive correlation was observed between glucose /protein oxidation, glucose/G‐actin and protein oxidation/G‐actin at 24 and 48 h. Our findings show that the oxidative effect of glucose on erythrocytes depends on concentration and incubation time. We also present the first evidence of increased G‐actin in human erythrocytes exposed to high glucose concentrations as a diabetes model. Copyright © 2004 John Wiley & Sons, Ltd.