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Tissue-Resident Macrophages Are Productively Infected Ex Vivo by Primary X4 Isolates of Human Immunodeficiency Virus Type 1

2005; American Society for Microbiology; Volume: 79; Issue: 8 Linguagem: Inglês

10.1128/jvi.79.8.5220-5226.2005

1098-5514

Prerana Jayakumar, Irina Berger, Frank Autschbach, Mark D. Weinstein, Benjamin Funke, Eric Verdin, Mark A. Goldsmith, Oliver T. Keppler,

HIV-related health complications and treatments

Infection of macrophages has been implicated as a critical event in the transmission and persistence of human immunodeficiency virus type 1 (HIV-1). Here, we explore whether primary X4 HIV-1 isolates can productively infect tissue macrophages that have terminally differentiated in vivo. Using immunohistochemistry, HIV-1 RNA in situ hybridization, and confocal immunofluorescence microscopy, we demonstrate that macrophages residing in human tonsil blocks can be productively infected ex vivo by primary X4 HIV-1 isolates. This challenges the model in which macrophage tropism is a key determinant of the selective transmission of R5 HIV-1 strains. Infection of tissue macrophages by X4 HIV-1 may be highly relevant in vivo and contribute to key events in HIV-1 pathogenesis.