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BIBTEX RIS

P04-45. Characterization of the plasma cell repertoire in acute HIV-1 infection (AHI)

2009; BioMed Central; Volume: 6; Issue: S3 Linguagem: Inglês

10.1186/1742-4690-6-s3-p73

ISSN

1742-4690

Autores

Lin Liao, X Chen, Angela Dixon, Supriya Munshaw, M. Anthony Moody, R Zhang, A Nagel, Andrew Foulger, K Derosa, Robert Parks, M. McParland, JF Whitesides, DJ Marshall, Joshua D. Amos, Yiping Yang, Feng Gao, M. Tracie Shea, David M. Margolis, Gary M. Shaw, Martin Markowitz, Thomas N. Denny, Garnett Kelsoe, Georgia D. Tomaras, Thomas B. Kepler, Barton F. Haynes,

Tópico(s)

HIV Research and Treatment

Resumo

Methods Blood PCs from three AHI subjects obtained approximately 17, 20 and 30 days after HIV-1 transmission were sorted into 96-well plates for amplification of VH and VL genes by RT/PCR. The isolated VH and VL genes were expressed as recombinant IgG1 mAbs in 293T cells by transfection using linear Ig expression cassettes, The specificity of produced mAbs was determined by ELISA and luminex bead immunoassays against a panel of HIV-1 and non-HIV-1 antigens.

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