A rapid qPCR method for genetic sex identification of Salmo salar and Salmo trutta including simultaneous elucidation of interspecies hybrid paternity by high‐resolution melt analysis
2014; Wiley; Volume: 84; Issue: 6 Linguagem: Inglês
10.1111/jfb.12401
ISSN1095-8649
AutoresMarc Anglès d’Auriac, Henning André Urke, Torstein Kristensen,
Tópico(s)Genetic diversity and population structure
ResumoThis study presents an improved duplex quantitative polymerase chain reaction ( qPCR ) method using the master sex‐determining gene sdY as a marker for simultaneous genetic sex identification of salmonids of the Salmo genus and paternity elucidation for Salmo salar × Salmo trutta hybrids. This method will provide a new, simple and economical molecular tool for ecological studies of these species as well as for aquaculture purposes.
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