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The role of xylulokinase in Saccharomyces cerevisiae xylulose catabolism

2000; Oxford University Press; Volume: 190; Issue: 1 Linguagem: Inglês

10.1111/j.1574-6968.2000.tb09259.x

1574-6968

Peter Richard, Mervi Toivari, Merja Penttilä,

Enzyme Structure and Function

Many yeast species have growth rates on D-xylulose of 25^130% of those on glucose, but for Saccharomyces cerevisiae this ratio is only about 6%.The xylulokinase reaction has been proposed to be the rate-limiting step in the D-xylulose fermentation with S. cerevisiae.Overexpression of xylulokinase encoding XKS1 stimulated growth on D-xylulose in a S. cerevisiae strain to about 20% of the growth rate on glucose and deletion of the gene prevented growth on D-xylulose and D-xylulose metabolism.We have partially purified the xylulokinase and characterised its kinetic properties.It is reversible and will also accept D-ribulose as a substrate.