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Mapping of Mitochondrial RNA-Protein Interactions by Digital RNase Footprinting

2013; Cell Press; Volume: 5; Issue: 3 Linguagem: Inglês

10.1016/j.celrep.2013.09.036

2639-1856

Ganqiang Liu, Tim R. Mercer, Anne-Marie J. Shearwood, Stefan J. Siira, Moira Hibbs, John S. Mattick, Oliver Rackham, Aleksandra Filipovska,

RNA Research and Splicing

Human mitochondrial DNA is transcribed as long polycistronic transcripts that encompass each strand of the genome and are processed subsequently into mature mRNAs, tRNAs, and rRNAs, necessitating widespread posttranscriptional regulation. Here, we establish methods for massively parallel sequencing and analyses of RNase-accessible regions of human mitochondrial RNA and thereby identify specific regions within mitochondrial transcripts that are bound by proteins. This approach provides a range of insights into the contribution of RNA-binding proteins to the regulation of mitochondrial gene expression.