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Identification of epigenetic DNA modifications with a protein nanopore

2010; Royal Society of Chemistry; Volume: 46; Issue: 43 Linguagem: Inglês

10.1039/c0cc02864a

1364-548X

Emma V. B. Wallace, David Stoddart, Andrew J. Heron, Ellina Mikhailova, Giovanni Maglia, Timothy J. Donohoe, Hagan Bayley,

Epigenetics and DNA Methylation

Two DNA bases, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (hmC), marks of epigenetic modification, are recognized in immobilized DNA strands and distinguished from G, A, T and C by nanopore current recording. Therefore, if further aspects of nanopore sequencing can be addressed, the approach will provide a means to locate epigenetic modifications in unamplified genomic DNA.