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Quantification of abscisic acid using liquid chromatography/thermospray ionization tandem mass spectrometry for selected reaction monitoring

1993; Wiley; Volume: 7; Issue: 12 Linguagem: Inglês

10.1002/rcm.1290071202

1097-0231

Masahiko Okamoto, Keníchi Takahashi, Tadashi Doi,

Plant Stress Responses and Tolerance

Abstract A liquid chromatography/thermospray ionization tandem mass spectrometry (LC/TSP‐MS/MS) method was developed for the quantification of abscisic acid (ABA) in crude plant extracts without derivatization. By this technique interfering chemical background ions are eliminated and a detection limit for ABA olf 0.2 ng is achieved. Reproducibility for quantification is attained by parallel reaction monitoring of ABA and added ABA‐d 6 used as an internal standard. The standard curve is linear over the range 200 pg to 10 ng of injected ABA. As examples of the application of the methodology, the quantification of ABA in some plant tissues is given.