Phagocytosed Bordetella pertussis Fails To Survive in Human Neutrophils
2000; American Society for Microbiology; Volume: 68; Issue: 2 Linguagem: Inglês
10.1128/iai.68.2.956-959.2000
ISSN1098-5522
AutoresDerrick H. Lenz, Christine L. Weingart, Alison A. Weiss,
Tópico(s)Infective Endocarditis Diagnosis and Management
ResumoABSTRACT Previous studies have reported that phagocytosed Bordetella pertussis survives in human neutrophils. This issue has been reexamined. Opsonized or unopsonized bacteria expressing green fluorescent protein (GFP) were incubated with adherent human neutrophils. Phagocytosis was quantified by fluorescence microscopy, and the viability of phagocytosed bacteria was determined by colony counts following treatment with polymyxin B to kill extracellular bacteria. Only 1 to 2% of the phagocytosed bacteria remained viable. Opsonization with heat-inactivated immune serum reduced the amount of attachment and phagocytosis of the bacteria but did not alter survival rates. In contrast to previous reports, these data suggest that phagocytosed B. pertussis bacteria are killed by human neutrophils.
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