A Minimally Invasive Tracer Protocol Is Effective for Assessing the Response of Leucine Kinetics and Oxidation to Vaccination in Chronically Energy-Deficient Adult Males and Children
1999; Elsevier BV; Volume: 129; Issue: 8 Linguagem: Inglês
10.1093/jn/129.8.1537
ISSN1541-6100
AutoresAnura V. Kurpad, Farook Jahoor, Sudhir Borgonha, Smita Poulo, Swarna Rekha, Carla R. Fjeld, Peter J. Reeds,
Tópico(s)Adipose Tissue and Metabolism
ResumoIn disadvantaged populations, recurrent infections lead to a loss of body nitrogen and worsen nutritional status. The resulting malnutrition, in its turn, produces a greater susceptibility to infection. This study aimed to examine the ability of a new minimally invasive tracer protocol to measure leucine oxidation, and then to use it to quantify the effect of vaccination on leucine kinetics and oxidation. Undernourished men (n = 5; body mass index 16.3 ± 0.9 kg/m2) and children (n = 9; age 4.1 ± 0.6 y; weight-for-age Z-score −2.3 ± 0.7) underwent metabolic studies 6 d before and 1 d after vaccination with diphtheria, pertussis and tetanus (DPT). The tracer protocol was performed in the fed state and involved two 3-h sequential periods of frequent (20 min) oral doses of NaH13CO3 or [1-13C] leucine. Frequent breath samples and urine collections were made. Blood samples were obtained from the men and used for the determination of the isotopic enrichment of α-ketoisocaproic acid. The prevaccination oxidation of leucine (percentage of dose ± SD) was 18.1 ± 2.3 (men) and 16.7 ± 3.8 (children). One day after vaccination, these values had risen to 19.9 ± 1.9 (P < 0.05) in the men and to 19.5 ± 4.6 (P < 0.01) in the children. In the adults, vaccination was associated with a rise in whole-body protein breakdown [mg protein/(kg·h)] from 200 ± 40 to 240 ± 10 (P < 0.05). A minor simulated infection increases leucine catabolism in undernourished humans and this new, minimally invasive protocol is sufficiently sensitive to measure these changes.
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