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Molecular Epidemiology of American Tegumentary Leishmaniasis in Panama

2009; American Society of Tropical Medicine and Hygiene; Volume: 81; Issue: 4 Linguagem: Inglês

10.4269/ajtmh.2009.08-0265

1476-1645

Aracelis Miranda, R. C. Carrasco, Hector Marino Pérez Paz, Juan Miguel Pascale, Franklyn Samudio, Azäel Saldaña, Giovanna Santamaría, Yaxelis Mendoza, Igor Calzada,

Parasites and Host Interactions

American tegumentary leishmaniasis is an increasing public health problem in Panama. This study describes the clinical characteristics and the molecular epidemiology of leishmaniasis in Panama over a 5-year period (2004–2008). Additionally, we applied a polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP)–based assay to identify Leishmania species in clinical isolates, skin scrapings, and sandflies specimens. Whereas 60.3% of cases were detected with conventional parasitologic techniques (smear or in vitro culture), the PCR detected 72% positive patients. Our clinical-epidemiologic data corroborate the high incidence of L. ( Viannia ) panamensis and provide evidence of peridomestic and/or domestic transmission. Mucosal involvement was observed in 4.2% of the patients. The overall natural infection rate with Leishmania in 103 pools of sandflies was 0.46%. Lutzomyia gomezi and Lutzomya panamensis were the prevalent species incriminated as vectors at the capture sites in central Panama. This study contributes to a better knowledge of the current epidemiology of tegumentary leishmaniasis in Panama.