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Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique

2015; Multidisciplinary Digital Publishing Institute; Volume: 15; Issue: 8 Linguagem: Inglês

10.3390/s150819819

1424-8220

María Fe Laguna Heras, Miguel Holgado, Ana Hernández Martínez, Beatriz Santamaría, Álvaro Lavín, Javier Millán Soria, Tatiana Suárez, Carlota Bardina, Mónica Jara, Francisco J. Sanza, Rafael Casquel,

Advanced Biosensing Techniques and Applications

The specificity and affinity of antibody-antigen interactions is a fundamental way to achieve reliable biosensing responses. Different proteins involved with dry eye dysfunction: ANXA1, ANXA11, CST4, PRDX5, PLAA and S100A6; were validated as biomarkers. In this work several antibodies were tested for ANXA1, ANXA11 and PRDX5 to select the best candidates for each biomarker. The results were obtained by using Biophotonic Sensing Cells (BICELLs) as an efficient methodology for label-free biosensing and compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) technique.