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A sandwich enzyme-linked immunosorbent assay for human seminal γ-glutamyl transpeptidase

1995; Elsevier BV; Volume: 76; Issue: 1 Linguagem: Inglês

10.1016/0379-0738(95)01794-1

1872-6283

Sumiko Abe, Hironori Gunji, Teizo Fujita, Kouichi Hiraiwa,

Bladder and Urothelial Cancer Treatments

A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for detecting human seminal gamma-glutamyl transpeptidase (gamma-GTP) using a combination of anti-seminal gamma-GTP monoclonal antibodies. These monoclonal antibodies did not react with human ovary or uterus in immunohistochemical study. Optimal assay condition, resulting in a sensitive assay with a low background, is presented. The detection limit of this assay was estimated to be 1 ng/ml of seminal gamma-GTP corresponding to about 100,000 times dilution of seminal sample. This ELISA was specific for seminal gamma-GTP, without cross-reactivity to renal or hepatic gamma-GTP, normal blood serum, non-coital vaginal fluid or saliva. The recovery of seminal gamma-GTP added to various biological fluids were also examined.