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Microfluidic device reads up to four consecutive base pairs in DNA sequencing-by-synthesis

2004; Oxford University Press; Volume: 32; Issue: 9 Linguagem: Inglês

10.1093/nar/gkh613

1362-4962

Emil P. Kartalov,

Electrowetting and Microfluidic Technologies

We have developed the first fully integrated microfluidic system for DNA sequencing‐by‐synthesis. Using this chip and fluorescence detection, we have reliably sequenced up to 4 consecutive bps. The described sequencer can be integrated with other microfluidic components on the same chip to produce true lab‐on‐a‐chip technology. The surface chemistry that was designed to anchor the DNA to elastomeric microchannels is useful in a broad range of studies and applications.