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Both CheA and CheW are required for reconstitution of chemotactic signaling in Escherichia coli

1989; American Society for Microbiology; Volume: 171; Issue: 9 Linguagem: Inglês

10.1128/jb.171.9.5190-5193.1989

1098-5530

Matthew P. Conley, A J Wolfe, David F. Blair, Howard C. Berg,

Protein Structure and Dynamics

If cells of Escherichia coli deleted for genes that specify transducers and all known cytoplasmic chemotaxis proteins are reconstituted with CheA, CheW, and CheY, they spin their flagella alternately clockwise and counterclockwise. If the aspartate receptor also is present, clockwise rotation is suppressed upon addition of aspartate. If either CheA or CheW is absent, the fraction of time that the flagella spin clockwise is reduced and responses to aspartate do not occur.