Produção Nacional
Revisado por pares
Effectiveness of three instrumentation systems to remove Enterococcus faecalis from root canals
2011; Wiley; Volume: 45; Issue: 5 Linguagem: Inglês
10.1111/j.1365-2591.2011.01994.x
ISSN1365-2591
AutoresManoel Matos Neto, Silvana Soléo Ferreira dos Santos, Mariella Vieira Pereira Leão, Sandramarcia Habitante, Jane Rose Dias Dionísio Rodrigues, Antônio Olavo Cardoso Jorge,
Tópico(s)Dental materials and restorations
ResumoTo assess the effectiveness of three systems of mechanical preparation to reduce Enterococcus faecalis within root canals.Twenty-four human single-rooted canine teeth were standardized to a length of 17 mm and the canal contents removed using a size 20 K-file, as the last apical file. After irrigation and sterilization, the canals were contaminated with E. faecalis and incubated for 21 days at 37 °C with 5% CO(2). Then, the teeth were divided into three groups for mechanical preparation with: ProTaper rotary system, ProTaper manual system and manual K-files. Samples of the root canal contents, before and after the debridement, were collected with sterile paper points for 1 min. Then, the samples were diluted and plated in Brain Heart Infusion (BHI) agar. The colony-forming units were counted and the percentage reduction calculated. The reduction and log CFU mL(-1) were compared between groups using Wilcoxon nonparametric test and two-way analysis of variance, respectively.There was a significant reduction in the number of CFU/mL (P = 0.000) before and after debridement for all the systems used. However, there was no significant difference between the systems.All the three instrumentation systems reduced E. faecalis counts to a similar degree.
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