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Rapid Identification of Aedes albopictus, Aedes scutellaris, and Aedes aegypti Life Stages Using Real-time Polymerase Chain Reaction Assays

2008; American Society of Tropical Medicine and Hygiene; Volume: 79; Issue: 6 Linguagem: Inglês

10.4269/ajtmh.2008.79.866

1476-1645

Lydia A. Hill, Joseph Davis, George Hapgood, Peter I Whelan, Gregory A. Smith, Scott A. Ritchie, Robert D. Cooper, Andrew F. van den Hurk,

Malaria Research and Control

In 2005, a widespread infestation of Aedes albopictus was discovered in the Torres Strait, the region between northern Australia and New Guinea. To contain this species, an eradication program was implemented in 2006. However, the progress of this program is impeded by the difficulty of morphologically separating Ae. albopictus larvae from the endemic species Aedes scutellaris. In this study, three real-time TaqMan polymerase chain reaction assays that target the ribosomal internal transcribed spacer 1 region were developed to rapidly identify Aedes aegypti, Ae. albopictus, and Ae. scutellaris from northern Australia. Individual eggs, larvae, pupae, and adults, as well as the species composition of mixed pools were accurately identified. The assay method was validated using 703 field-collected specimens from the Torres Strait.