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BIBTEX RIS

A simple method for human peripheral blood monocyte Isolation

2000; Instituto Oswaldo Cruz, Ministério da Saúde; Volume: 95; Issue: 2 Linguagem: Inglês

10.1590/s0074-02762000000200014

ISSN

1678-8060

Autores

Marcos Célio de Almeida, Alan C. Silva, Aldina Barral, Manoel Barral‐Netto,

Tópico(s)

Cell Adhesion Molecules Research

Resumo

We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.

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