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Genotoxicity Induced by Eugenia Caryophyllata Infusion

2008; Taylor & Francis; Volume: 71; Issue: 7 Linguagem: Inglês

10.1080/15287390701839232

1087-2620

Patrícia Estevam dos Santos, Lucila Carmem Monte Egito, Sílvia Regina Batistuzzo de Medeiros, Lucymara Fassarella Agnez‐Lima,

Plant tissue culture and regeneration

Abstract Several therapeutic properties have been described for Eugenia caryophyllata (clove). In the present study the infusion of E. caryophyllata was evaluated in a series of bacterial and cell-free assays in order to determine genotoxic potential. Negative results were obtained in the SOS chromotest and in the Salmonella reversion assay using strains TA97a, TA98, TA100, and TA102. However, in a forward mutagenesis assay an increase in mutagenesis and high cytotoxicity was observed with the CC104 mutMmutY strain, suggesting that oxidative DNA damage occurred. The treatment of plasmid with clove infusion showed that DNA strand breaks and sites recognized by formamidopyrimidine-DNA-glycosylase (FPG/MutM) were generated. Data suggest that the occurrence of oxidative DNA damage, with low mutagenic potential, may also be involved in the cytotoxicity attributed to clove infusion. We thank Dr. Alvaro Costa Leitão (from UFRJ, Brazil) and Dr. Gisela de Aragão Umbuzeiro (from CETESB, Brazil) for providing some bacterial strains and Glenn Haves (from the American Language Program of the University of Georgia) for editing this article. We also thank CNPq and Fundação de Amparo a Pesquisa em Alimentos e Medicamentos da Universidade Federal do Rio Grande do Norte (FAPAM-UFRN) for financial support. P. E. dos Santos received grants from CNPq, and L. F. Agnez-Lima has a CNPq fellowship.