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Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion

2009; National Academy of Sciences; Volume: 106; Issue: 6 Linguagem: Inglês

10.1073/pnas.0809575106

1091-6490

Diego Martínez, Jean F. Challacombe, Ingo Morgenstern, David S. Hibbett, Monika Schmoll, Christian P. Kubicek, Patrícia Ferreira, Francisco J. Ruiz‐Dueñas, Ángel T. Martı́nez, Phil Kersten, Kenneth E. Hammel, Amber Vanden Wymelenberg, Jill Gaskell, Erika Lindquist, Grzegorz Sabat, Sandra Splinter BonDurant, Luis Larrondo, Paulo Canessa, Rafael Vicuña, Jagjit S. Yadav, HarshaVardhan Doddapaneni, Venkataramanan Subramanian, Antonio G. Pisabarro, José Luis Lavín, José A. Oguiza, Emma R. Master, Bernard Henrissat, Pedro M. Coutinho, Paul V. Harris, Jon Magnuson, Scott Baker, Kenneth S. Bruno, William R. Kenealy, Patrik J. Hoegger, Ursula Kües, Preethi Ramaiya, Susan Lucas, Asaf Salamov, Harris Shapiro, Hank Tu, Christine Chee, Monica Misra, Gary Xie, Sarah Teter, Debbie Yaver, Tim Y. James, Martin Mokrejš, Martin Pospíšek, Igor V. Grigoriev, Thomas Brettin, Dan Rokhsar, Randy M. Berka, Dan Cullen,

Biofuel production and bioconversion

Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood. Systematic examination of the P. placenta genome, transcriptome, and secretome revealed unique extracellular enzyme systems, including an unusual repertoire of extracellular glycoside hydrolases. Genes encoding exocellobiohydrolases and cellulose-binding domains, typical of cellulolytic microbes, are absent in this efficient cellulose-degrading fungus. When P. placenta was grown in medium containing cellulose as sole carbon source, transcripts corresponding to many hemicellulases and to a single putative β-1–4 endoglucanase were expressed at high levels relative to glucose-grown cultures. These transcript profiles were confirmed by direct identification of peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Also up-regulated during growth on cellulose medium were putative iron reductases, quinone reductase, and structurally divergent oxidases potentially involved in extracellular generation of Fe(II) and H 2 O 2 . These observations are consistent with a biodegradative role for Fenton chemistry in which Fe(II) and H 2 O 2 react to form hydroxyl radicals, highly reactive oxidants capable of depolymerizing cellulose. The P. placenta genome resources provide unparalleled opportunities for investigating such unusual mechanisms of cellulose conversion. More broadly, the genome offers insight into the diversification of lignocellulose degrading mechanisms in fungi. Comparisons with the closely related white-rot fungus Phanerochaete chrysosporium support an evolutionary shift from white-rot to brown-rot during which the capacity for efficient depolymerization of lignin was lost.