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A new technique for obtaining thin lipid films separating two aqueous media

1965; Elsevier BV; Volume: 12; Issue: 1 Linguagem: Inglês

10.1016/s0022-2836(65)80302-3

1089-8638

H.J. van den Berg,

Analytical Chemistry and Sensors

A novel method to construct a stable and uniform phospholipid membrane of large area and good manipulability is reported. Using the Langmuir-Blodgett (LB) technique, a monolayer of phospholipid can be transferred to filter paper. The electrical conductance across the pores of the lipid membrane is about 1.8×10−9 S/cm2, corresponding to the conductance of 10−7–10−10 S/cm2 reported for bilayer lipid membranes (BLM) of phospholipids. A scanning electron micrograph demonstrated that the phospholipid membrane on the filter paper was uniform.