Portal de Periódicos da CAPES

Oxygen affinity and stability of hemoglobin dunn (α6(A4)Asp→Asn): Use of isoelectric focusing in recognition of a new abnormal hemoglobin

1980; Wiley; Volume: 9; Issue: 2 Linguagem: Inglês

10.1002/ajh.2830090203

1096-8652

Samuel Charache, Bernadine Brimhall, Ghazi Zaatari, P. Hathaway, I. Steiman,

Erythrocyte Function and Pathophysiology

Abstract A new slow‐moving hemoglobin was found in low proportion in an asymptomatic black woman. Isoelectric focusing helped to distinguish it from other hemoglobins with similar electrophoretic mobility, and amino acid analysis showed that aspartic acid α6 (A4) had been replaced by asparagine. Oxygen affinity was increased, but the Bohr and DPG effects were normal. Stability of the purified hemoglobin was decreased, but that of hemolysates was normal. Abnormal oxygen affinity of this variant, and that of hemoglobin Sawara (α6(A4)Asp→Ala), may reflect loss of a salt bridge between Asp α6 and Lys‐α127(H10) which would tend to favor the high‐affinity R conformation of the molecule.