Preparation and microbial decomposition of synthetic [14C]ligins.
1975; National Academy of Sciences; Volume: 72; Issue: 7 Linguagem: Inglês
10.1073/pnas.72.7.2515
ISSN1091-6490
AutoresT. Kent Kirk, William Connors, R. D. Bleam, W. F. Hackett, J. G. Zeikus,
Tópico(s)Biochemical and biochemical processes
ResumoA definitive assay for microbiological and biochemical research on the biodegradation of lignin was developed using radioactive synthetic lignins specifically labeled in the side chains, aromatic rings or in the methoxyl groups. The [14C]lignins were prepared by oxidative polymerization with peroxidase and H2O2 Of specifically labeled coniferyl alcohol (4-hydroxy-3-methyoxycinnamyl alcohol). The synthetic polymers were shown by spectroscopic and chemical methods to contain the same intermonomer linkages found in natural lignins. Incubation of the [14C]lignins with known lignin-degrading fungi and with a forest soil resulted in 14CO2 evolution.
Referência(s)