The Isopaque–Ficoll Method Re‐evaluated: Selective Loss of Autologous Rosette‐forming Lymphocytes during Isolation of Mononuclear Cells from Human Peripheral Blood
1980; Wiley; Volume: 11; Issue: 3 Linguagem: Inglês
10.1111/j.1365-3083.1980.tb00245.x
ISSN1365-3083
Autores Tópico(s)Blood groups and transfusion
ResumoA subpopulation of lymphocytes is defined, which—under conditions used for Isolating mono‐nuclear cells from peripheral blood by the Isopaque‐Ficoll gradient—is lost in the erythrocyte pellet because of rosette formation with the autologous erythrocytes in the gradient. Reapplication of the resuspended erythrocyte pellet on a new gradient with higher density could, however, recover the subpopulation. Lymphocyte surface marker analysis on such cell showed that they were enriched in T cell (judged by rosette formation with sheep erythrocytes) and in auto‐rosette‐forming cells. The importance of these findings is discussed in relation to the wide use of the Isopaque‐Ficoll method.
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