Portal de Periódicos da CAPES

BLADDER Aδ AFFERENT NERVE ACTIVITY IN NORMAL CATS AND CATS WITH FELINE INTERSTITIAL CYSTITIS

2005; Lippincott Williams & Wilkins; Volume: 173; Issue: 3 Linguagem: Inglês

10.1097/01.ju.0000145591.35569.9e

1527-3792

James R. Roppolo, Changfeng Tai, August M. Booth, C. A. Tony Buffington, William C. de Groat, Lori A. Birder,

Pediatric Urology and Nephrology Studies

No AccessJournal of UrologyInvestigative Urology1 Mar 2005BLADDER Aδ AFFERENT NERVE ACTIVITY IN NORMAL CATS AND CATS WITH FELINE INTERSTITIAL CYSTITIS JAMES R. ROPPOLO, CHANGFENG TAI, AUGUST M. BOOTH, C.A. TONY BUFFINGTON, WILLIAM C. de GROAT, and LORI A. BIRDER JAMES R. ROPPOLOJAMES R. ROPPOLO , CHANGFENG TAICHANGFENG TAI , AUGUST M. BOOTHAUGUST M. BOOTH , C.A. TONY BUFFINGTONC.A. TONY BUFFINGTON , WILLIAM C. de GROATWILLIAM C. de GROAT , and LORI A. BIRDERLORI A. BIRDER View All Author Informationhttps://doi.org/10.1097/01.ju.0000145591.35569.9eAboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Symptoms associated with interstitial cystitis (IC) (urinary frequency, urgency and pain) resemble those in cats diagnosed with feline IC (FIC). Patients with IC also show enhanced sensitivity to intravesical application of KCl solution. In the current study we compared the effect of bladder distention and intravesical infusion of KCl solution on Aδ bladder afferent nerve activity in normal and FIC cats. Materials and Methods: Cats were anesthetized with α-chloralose (60 to 70 mg/kg intravenously). Single and multiunit afferent nerve activity was recorded with platinum electrodes from fibers dissected from the transected pelvic nerve. Bladder Aδ afferents were activated by bladder distention at constant pressures (5 to 80 cm H2O) by infusing saline or KCl solution (80 to 300 mM). Results: Increasing bladder pressure by saline infusion over a range of constant pressures (5 to 80 cm H2O) increased afferent firing in normal and FIC cats. However, the slope of the pressure-firing curve was significantly steeper in cats with FIC. In normal cats KCl infusion (80 to 300 mM) into the bladder produced a pressure-firing curve similar to that induced by saline infusion. However, in FIC cats KCl at concentrations of 150 to 300 mM produced almost complete inhibition of afferent firing at pressures between 30 and 80 cm H2O. Conclusions: These data suggest that 1) bladder Aδ afferents in FIC cats are more sensitive to pressure changes than are afferents in normal cats and 2) the inhibition of afferent firing by high concentrations of KCl in FIC cats is likely the result of afferent nerve depolarization and it is consistent with the idea of increased permeability of bladder epithelium in FIC. References 1 : Interstitial Cystitis. Philadelphia: Lippincott-Raven1997. Google Scholar 2 : Interstitial cystitis and related diseases. In: . Philadelphia: W. B. Saunders Co.1998: 631. sect. IV, chapt. 17. Google Scholar 3 : Comorbidity of interstitial cystitis with other unexplained clinical conditions. J Urol2004; 172: 1242. Google Scholar 4 : In vivo models of interstitial cystitis. J Urol2002; 167: 694. Link, Google Scholar 5 : Summary of the National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases workshop on interstitial cystitis. J Urol1988; 140: 203. Link, Google Scholar 6 : The diagnosis of interstitial cystitis revisited: lessons learned from the National Institutes of Health Interstitial Cystitis Database Study. J Urol1999; 161: 553. Link, Google Scholar 7 : Distinctive ultrastructural pathology of nonulcerative interstitial cystitis: new observations and their potential significance in pathogenesis. Urol Int1996; 56: 137. Google Scholar 8 : Chronic interstitial cystitis: a heterogeneous syndrome. J Urol1987; 137: 35. Link, Google Scholar 9 : Modulation of epithelial permeability by extracellular macromolecules. Physiol Rev1995; 75: 561. Google Scholar 10 : Bladder surface glycosaminoglycans: an epithelial permeability barrier. J Urol1990; 143: 139. Link, Google Scholar 11 : Epithelial dysfunction in nonbacterial cystitis (interstitial cystitis). J Urol1991; 145: 732. Link, Google Scholar 12 : Urothelial pathophysiological changes in feline interstitial cystitis: a human model. Am J Physiol Renal Physiol2000; 278: F540. Crossref, Medline, Google Scholar 13 : Abnormal sensitivity to intravesical potassium in interstitial cystitis and radiation cystitis. Neurourol Urodyn1994; 13: 515. Google Scholar 14 : Bladder afferent neural activity in both normal and cats with feline interstitial cystitis (FIC). Washington, D. C.: Society for Neuroscience2002. abstract. Google Scholar 15 : Myelinated primary afferents of the sacral spinal cord responding to slow filling and distension of the cat urinary bladder. J Physiol1993; 463: 449. Google Scholar 16 : Receptive properties of myelinated primary afferents innervating the inflamed urinary bladder of the cat. J Neurophysiol1993; 69: 395. Crossref, Medline, Google Scholar 17 : Activation of unmyelinated afferent fibres by mechanical stimuli and inflammation of the urinary bladder in the cat. J Physiol1990; 425: 545. Google Scholar 18 : On the function of spinal primary afferent fibres supplying colon and urinary bladder. J Autonom Nerv Syst1990; 30: S89. Google Scholar 19 : Development of chronic renal disease in cats fed a commercial diet. J Am Vet Med Assoc1993; 202: 744. Google Scholar From the Departments of Pharmacology (JRR, CT, AMB, WCdG, LAB) and Medicine (LAB), University of Pittsburgh, Pittsburgh, Pennsylvania, and Department of Veterinary Clinical Sciences, Ohio State University (CATB), Columbus, Ohio© 2005 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 173Issue 3March 2005Page: 1011-1015 Advertisement Copyright & Permissions© 2005 by American Urological Association, Inc.Keywordsbladdercatsafferent pathwayscystitis, interstitialMetrics Author Information JAMES R. ROPPOLO More articles by this author CHANGFENG TAI More articles by this author AUGUST M. BOOTH More articles by this author C.A. TONY BUFFINGTON More articles by this author WILLIAM C. de GROAT Financial interest and/or other relationship with Abbott Pharmaceutical, Roche Palo Alto, Eli Lilly Pharmaceutical, Pfizer Pharmaceutical, Novartis Pharmaceutical, Dynogen, Johnson and Johnson Pharmaceutical, Urogene, Newron, ICOS and Neurogen. More articles by this author LORI A. BIRDER Financial interest and/or other relationship with Roche Palo Alto. More articles by this author Expand All Advertisement PDF downloadLoading ...