Produção Nacional
Revisado por pares
Trypanosoma cruzi: identification of specific epimastigote antigens by human immune sera
1989; Instituto Oswaldo Cruz, Ministério da Saúde; Volume: 84; Issue: 3 Linguagem: Inglês
10.1590/s0074-02761989000300004
ISSN1678-8060
AutoresM. G. Morgado, J. Ivo-dos-Santos, Rosa T. Pinho, Eduardo Arguelles, Joffre M. Rezende, Bernardo Galvão‐Castro,
Tópico(s)Trypanosoma species research and implications
ResumoSoluble antigens from epimastigotes of Trypanosoma cruzi were analyzed by western blot in terms of their reactivity with sera from patients with Chagas' disease. In addition, sera from patients with visceral (AVL) and tegumental leishmaniasis (ATL) were also tested in order to identify cross-reactivities with Trypanosoma cruzi antigens. Twenty eight polypeptides with molecular weights ranging from 14 kDa to 113 kDa were identified with sera from Chagas' disease patients. An extensive cross-reactivity was observed when sera from human visceral leishmaniasis were used, while only a slight cross-reaction was observed with sera from tegumental leishmaniasis. On the other hand, 10 polypeptides specifically reacting with sera from Chagas' disease patients were identified. Among them, the antigens with molecular weights of 46 kDa and 25 kDa reacted with all sera tested and may be good candidates for specific immunodiagnosis of Chagas' disease.
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