Crystallographic Analysis of the Recognition of a Nuclear Localization Signal by the Nuclear Import Factor Karyopherin α
1998; Cell Press; Volume: 94; Issue: 2 Linguagem: Inglês
10.1016/s0092-8674(00)81419-1
ISSN1097-4172
AutoresElena Conti, Marc Uy, L. Leighton, Günter Blobel, John Kuriyan,
Tópico(s)Heat shock proteins research
ResumoSelective nuclear import is mediated by nuclear localization signals (NLSs) and cognate transport factors known as karyopherins or importins. Karyopherin α recognizes “classical” monopartite and bipartite NLSs. We report the crystal structure of a 50 kDa fragment of the 60 kDa yeast karyopherin α, in the absence and presence of a monopartite NLS peptide at 2.2 Å and 2.8 Å resolution, respectively. The structure shows a tandem array of ten armadillo repeats, organized in a right-handed superhelix of helices. Binding of the NLS peptide occurs at two sites within a helical surface groove that is lined by conserved residues. The structure reveals the determinants of NLS specificity and suggests a model for the recognition of bipartite NLSs.
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