Produção Nacional
Revisado por pares
Effects of a novel calcium aluminate cement on the early events of the progression of osteogenic cell cultures
2011; Fundação Odontológica de Ribeirão Preto; Volume: 22; Issue: 2 Linguagem: Inglês
10.1590/s0103-64402011000200002
ISSN1806-4760
AutoresLarissa Moreira Spinola de Castro Raucci, I. R. Oliveira, Lucas Novaes Teixeira, Adalberto Luiz Rosa, Paulo Tambasco de Oliveira, Marcos Jacobovitz,
Tópico(s)Drilling and Well Engineering
ResumoThe present study evaluated the progression of osteogenic cell cultures exposed to a novel calcium aluminate cement (CAC+) in comparison with the gold standard mineral trioxide aggregate (MTA). Cells were enzimatically isolated from newborn rat calvarial bone, plated on glass coverslips containing either CAC+ or a control MTA samples in the center, and grown under standard osteogenic conditions. Over the 10-day culture period, roundening of sample edges was clearly noticed only for MTA group. Although both cements supported osteogenic cell adhesion, spreading, and proliferation, CAC+-exposed cultures showed significantly higher values in terms of total cell number at days 3 and 7, and total protein content and alkaline phosphatase activity at day 10. The present in vitro results indicate that the exposure to CAC+ supports a higher differentiation of osteogenic cells compared with the ones exposed to MTA. Further experimental studies should consider CAC+ as a potential alternative to MTA when the repair of mineralized tissues is one of the desired outcomes in endodontic therapy.
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