Produção Nacional
Revisado por pares
Sequence Similarity-Based Proteomics in Insects: Characterization of the Larvae Venom of the Brazilian Moth Cerodirphia s peciosa
2005; American Chemical Society; Volume: 4; Issue: 3 Linguagem: Inglês
10.1021/pr0500051
ISSN1535-3907
AutoresAnna Shevchenko, Mirta Mittelstedt Leal de Sousa, Patrice Waridel, Silvia Tolfo Bittencourt, Marcelo Valle de Sousa, Andriy Shevchenko,
Tópico(s)Venomous Animal Envenomation and Studies
ResumoUsing a combination of tandem mass spectrometric sequencing and sequence similarity searches, we characterized the larvae venom of the moth Cerodirphia speciosa, which belongs to the Saturniidae family of the Lepidoptera order. Despite the paucity of available database sequence resources, the approach enabled us to identify 48 out of 58 attempted spots on its two-dimensional gel electrophoresis map, which represented 37 unique proteins, whereas it was only possible to identify 13 proteins by conventional non-error tolerant database searching methods. The majority of cross-species hits were made to proteins from the phylogenetically related Lepidoptera organism, the silk worm Bombyx mori. The protein composition of the venom suggested that envenoming by C. speciosa toxins might proceed through the contact with its hemolymph, similarly to another toxic Lepidoptera organism, Lonomia obliqua.
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