Efecto del consumo de la fibra dietética en la expresión cuantitativa del receptor de butirato GPR43 en colon de ratas
2011; Arán Ediciones; Volume: 26; Issue: 5 Linguagem: Inglês
10.3305/nh.2011.26.5.5054
ISSN1699-5198
AutoresL. Y. Corte Osorio, Héctor Eduardo Martínez‐Flores, Rafael Ortíz‐Alvarado,
Tópico(s)Botanical Research and Applications
ResumoIntroduction: Short chain fatty acids (SCFA) acetate, propionate and butyrate are the major anions produced by the bacterial fermentation of dietary fiber (DF) in colon. Recently, butyrate has been recently studied because is important to maintain colonic functions and because it has been related with a protective effect in colorectal cancer, which is mainly, explained by its potential to regulate gene expression by inhibiting enzyme histone deacetylase (HDAC). Several investigations shown that SCFA receptor GPR43 is involved in signal transduction mechanisms once they bind to ligands such as butyrate to generate different physiological effects in colonocytes. Objective: Determine if dietary fiber consumption from nopal (Opuntia ficus I.) containing a ratio of soluble-insoluble fiber 40/60, has a direct influence on the quantitative expression of butyrate-specific receptor GPR43. Methods: Wistar rats were fed with four different diets formulated at different concentrations of dietary fiber of 0, 5, 15 and 25% of dietary fiber from opuntia, respectively. Results and discussion: The results shown an increase in the expression of GPR43 (93.1%) when rats was fed with a 5% fiber diet, using β-actin as a reference gene. The results of this investigation will contribute to determinate the relation of diet with intestinal health for the purpose of expanding the knowledge of butyric acid on colonic functions.
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