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Comparison of Three Solvent Systems for Extraction of Chlorophyll a from Fish Pond Phytoplankton Communities

1988; Wiley; Volume: 19; Issue: 2 Linguagem: Inglês

10.1111/j.1749-7345.1988.tb01043.x

1749-7345

Steven W. Lloyd, Craig S. Tucker,

Water Quality Monitoring and Analysis

Abstract Chloroform‐methanol (2:1 v/v), absolute methanol, and 90% acetone were evaluated for their effectiveness as extractants of chlorophyll a from samples of phytoplankton communities collected from catfish ponds. Chloroform‐methanol consistently extracted more chlorophyll a than either 90% acetone or methanol. Precision for the methanol extraction was also unacceptably low, with an average coefficient of variation of 17%. Average coefficients of variation for the chloroform‐methanol and 90% acetone extraction procedures were 6 and 5%, respectively. Filtered samples should be steeped in chloroform‐methanol for at least 4 h to obtain maximum chlorophyll extraction, and the addition of MgCO 3 to the extractant as a buffer is not necessary.