Taking EPR "Snapshots" of the Oxidative Stress Status in Human Blood
2003; Taylor & Francis; Volume: 37; Issue: 5 Linguagem: Inglês
10.1080/1071576031000076240
ISSN1071-5762
AutoresMoreno Paolini, Luca Valgimigli, Emanuela Marchesi, Silvia Trespidi, Gian Franco Pedulli,
Tópico(s)Coenzyme Q10 studies and effects
ResumoAbstractAssessment of oxidative stress status (OSS) in human tissues is still troublesome. Using an innovative EPR-radical-probe we successfully measured the instantaneous concentration of ROS directly in peripheral blood of athletes and normally active workers during 60 min controlled exercise. The probe employed was bis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decandioate, which quantitatively and instantaneously reacts with oxygen-centered radicals (including superoxide) to yield the parent nitroxide, which is sufficiently persistent to be measured by EPR. Our measurements suggest that while at rest normally active individuals may benefit more from antioxidant supplementation than athletes; conversely, during exercise athletes may benefit more from supplementation. Our method allows reliable, quick, and non-invasive quantitative determination of OSS in human peripheral blood.KeywordsEprFree RadicalsOxidative StressHuman BloodSport
Referência(s)