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Direct Application of the INNO-LiPA Rif.TB Line-Probe Assay for Rapid Identification of Mycobacterium tuberculosis Complex Strains and Detection of Rifampin Resistance in 360 Smear-Positive Respiratory Specimens from an Area of High Incidence of Multidrug-Resistant Tuberculosis

2005; American Society for Microbiology; Volume: 43; Issue: 9 Linguagem: Inglês

10.1128/jcm.43.9.4880-4884.2005

1098-660X

Miguel Viveiros, Clara Leandro, Liliana Rodrigues, J. M. Das Neves Almeida, Rosário Bettencourt, Isabel Couto, Lurdes Carrilho, José Diogo, Ana Mafalda Fonseca, Luís Lito, João Lopes, Teresa Pacheco, Mariana Pessanha, Judite Quirim, Luísa Sancho, Max Salfinger, Leonard Amaral,

Pneumonia and Respiratory Infections

ABSTRACT The INNO-LiPA Rif.TB assay for the identification of Mycobacterium tuberculosis complex strains and the detection of rifampin (RIF) resistance has been evaluated with 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis (MDR-TB). The sensitivity when compared to conventional identification/culture methods was 82.2%, and the specificity was 66.7%; the sensitivity and specificity were 100.0% and 96.9%, respectively, for the detection of RIF resistance. This assay has the potential to provide rapid information that is essential for the effective management of MDR-TB.