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Multiplex PCR Method for Detection of Clostridium difficile tcdA, tcdB , cdtA , and cdtB and Internal In-Frame Deletion of tcdC

2011; American Society for Microbiology; Volume: 49; Issue: 12 Linguagem: Inglês

10.1128/jcm.05161-11

1098-660X

Søren Persson, Joan N. Jensen, Katharina E. P. Olsen,

Microscopic Colitis

ABSTRACT A multiplex PCR method was developed for the detection of Clostridium difficile toxin genes tcdA , tcdB , ctdA , and cdtB and the major in-frame deletion types (18, 39, and 54 bp) of tcdC . The method has high specificity for PCR ribotype 027 and may identify other C . difficile strains of clinical and epidemiological importance.