Portal de Periódicos da CAPES

A rapid RP‐HPTLC densitometry method for simultaneous determination of major flavonoids in important medicinal plants

2007; Wiley; Volume: 30; Issue: 13 Linguagem: Inglês

10.1002/jssc.200700066

1615-9314

Pamita Bhandari, Neeraj Kumar, A. Gupta, Bikram Singh, Vijay Kumar Kaul,

Essential Oils and Antimicrobial Activity

Abstract A simple, sensitive, selective, precise, and robust high‐performance TLC (HPTLC) method was developed and validated for determination of flavonoids in herbal extracts Bauhinia variegata , Bacopa monnieri , Centella asiatica , Ginkgo biloba , Lonicera japonica , Rosa bourboniana , Rosa brunonii , and Rosa damascena. The HPTLC of flavonoids was performed on RP‐18 F 254 TLC plates with dual run, water (5% formic acid)/methanol (70:30) and water (5% formic acid)/methanol (50:50) as mobile phases. Densitometric determination of flavonoids was performed at λ = 280 nm in reflectance/absorbance mode. The linear regression analysis data for the calibration plots showed a good linear relationship with r 2 = 0.998 ± 0.0003 in the concentration range of 150–800 ng/spot for apigenin and rutin and 200–1000 ng/spot for quercetin, luteolin, and quercitrin with respect to peak area. The average recovery for apigenin, quercetin, rutin, luteolin, and quercitrin was 97–99.8% indicating the excellent reproducibility. Statistical analysis of the data showed that the method is reproducible and selective for determination of flavonoids.