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Anthraquinone production by hairy root culture of Rubia akane Nakai: influence of media and auxin treatment.

2009; Academic Journals; Volume: 4; Issue: 7 Linguagem: Inglês

10.5897/sre.9000186

1992-2248

Sang Un Park, Sook‐Young Lee,

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Hairy root culture of Rubia akane was studied using different media supplemented with different concentration of auxins for the growth and anthraquinones production. Hairy roots grown in full-strength SH medium showed the highest levels of root growth (12.2 g/L) as well as the highest amount of alizarin (4.5 mg/g D.W.) and purpurin production (5.5 mg/g D.W.) as compared to Rubia akane hairy roots grown in half- and full-strength B5 and MS medium, and half-strength SH medium. Among the different auxins treatment in hairy root culture, NAA performed the best both in growth as well as alizarin and purpurin production. With the concentration of 0.5mg/L NAA produced the highest amount of dry weight (14.2g/L) and the highest amount of both alizarin (5.9 mg/g D.W.) and purpurin (7.2 mg/g D.W.). These findings indicate that hairy root cultures of Rubia akane growing on SH media supplemented with 0.5 mg/L NAA could be a valuable alternative approach for anthraquinone production. Key words: Agrobacterium rhizogenes, anthraquinones, auxin, medium, hairy root culture, Rubia akane nakai.