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Cloning and sequencing of dolphinfish ( Coryphaena hippurus, Coryphaenidae ) growth hormone-encoding cDNA

1994; Taylor & Francis; Volume: 5; Issue: 2 Linguagem: Inglês

10.3109/10425179409039713

1042-5179

Adi Peduel, Abigail Elizur, Wayne Knibb,

RNA modifications and cancer

The cDNA encoding the preprotein growth hormone from the dolphinfish (Coryphaena hippurus) has been cloned and sequenced. The cDNA was derived by reverse trancription of RNA from the pituitary of a young fish using the method known as Rapid Amplification of cDNA Ends (RACE). An oligonucleotide primer corresponding to the 5′ region of Pagrus major and the universal RACE primer enabled amplification using the Polymerase Chain Reaction (PCR). The dolphinfish and yellow-tail, Seriola quineqeradiata, are both members of the sub-order Percoidei (Perciforme) and their GH sequences show a high level of homology.